Vitellogenesis and choriogenesis in fishes springerlink. Therefore, we suggest that zebrafish skin and eye tissues may be alternatives to plasma and liver tissues for vtg. Shortterm treatment of adult male zebrafish danio rerio with 17. Direct induction of vitellogenin synthesis in tissue culture for a long time, all attempts to elicit in vitro the full physiological response of a normal adult tissue. Due to the high expression levels of vtg and the ease of taking samples, skin and eye tissues were chosen to evaluate the effects of varying doses and exposure times of ee2 on male zebrafish. Large volumes of lymph can be collected from the eyesacs of bubbleeye goldfish. Vitellogenesis article about vitellogenesis by the free. An international group of researchers led by dr dalial freitak from the university of helsinki, finland has found that a bee blood protein called vitellogenin plays a critical role in. The transcription of the different zebrafish genes was analyzed by. Characteristics of fish yolk proteins and a method for. The results showed that the amount of vtg produced in plasma of exposed fish increased in logarithmic order comparing to the control group and the elisa described here could be used as an indicator of water pollution to estrogenic pollutants.
This study describes the development of quantitative polymerase chain reaction qpcr assays to characterize the expression of two vitellogenin vtg genes in the invasive round goby neogobius melanostomus. Vitellogenin drosophila lifespan or fecundity version 1. Bioassays play a central role in effectdirected analysis eda, and their selection and application have to consider rather specific aspects of this approach. Mar 27, 2007 the zebrafish, danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. Pdf temporal dynamics of oocyte growth and vitellogenin gene. Vitellogenin gene expression 3 tion process, as well as to study some aspects of the utilization of mrna for vitellogenin. Zebrafish as a model for studying ovarian development.
Specific primers were designed to amplify ash vg gene by polymerase chain reaction pcr and the obtained dna sequence was 7329 bp long, including 6 exons and 5 introns with an open reading frame encoding a 1774 amino acids peptide. The zebrafish, danio rerio, has emerged as a major model organism for biomedical research, yet little is known about its natural history. Stromqvist m, tooke n, brunstrom b 2010 dna methylation levels in the 5flanking region of the vitellogenin i gene in liver and brain of adult zebrafish danio reriosex and tissue differences and effects of 17aethinylestradiol exposure. Vitellogenin definition of vitellogenin by medical dictionary.
Apr 28, 2014 variable gene expression in zebrafish by karlsruhe institute of technology the zebrafish is used as a model organism for research into the embryonic development of vertebrates. Revealing genes associated with vitellogenesis in the liver of the. Immunohistochemical staining for vitellogenin in the liver of a male zebrafish exposed to 1 nm e2 right, as well as a control male not exposed, left. Characterization of vitellogenin gene expression in round. The vitellogenin gene family is constituted of variable gene numbers encoding for polypeptides that are precursors of yolk proteins and derivatives in oviparous and. Here the authors report that zebrafish embryos without maternally provided vg1 fail to form endoderm and head and trunk mesoderm, and closely resemble nodal lossoffunction mutants.
The zebrafish gene collection zgc is an nih initiative that supports the production of cdna libraries, clones and sequences to provide a complete set of fulllength open reading frame sequences and cdna clones of expressed genes for zebrafish. Vitellogenin is the precursor molecule for egg yolk, which is the source of metabolic energy for the developing embryo. Vitellogenin definition of vitellogenin by medical. The vitellogenin protein is produced by the adult insect fat body, which in honeybees consists of thin layers of cells spread against the body wall of the abdomen. Vitellogenesis starts in the relatively late stages of oogenesis, toward the end of which the size of the. These vitellogenin est clones have been derived from at least seven distinct vitellogenin genes. Hepatic and extrahepatic expression of vitellogenin genes. Vitellogenin is present in the cytoplasm of hepatocytes in a perinuclear area reminiscent of rergolgi arrows. Transcriptomic analyses of sexual dimorphism of the zebrafish. Pdf functions of vitellogenin in eggs researchgate.
Loss of egg yolk genes in mammals and the origin of. Red zebra danio glofishglow in the dark product view. We also report our own field study to document several aspects of zebrafish natural history across sites in northeast india. Pdf abstract little is known about how hepatic vitellogenin gene vtg expression relates to. The coding sequence for this gene is the most similar among all drosophila genes to vgencoding genes in honey bee 37% similarity, c. Detection of vitellogenin incorporation into zebrafish.
Guide for the laboratory use of zebrafish danio rerio 3rd edition university of oregon press. Youll need a nursery to raise your fry to adulthood. Variable gene expression in zebrafish by karlsruhe institute of technology the zebrafish is used as a model organism for research into the embryonic development of vertebrates. Characterization and expression profile of vitellogenin. In the female liver, the top four transcripts were all vitellogenin genes, including vtg1, vtg4, vtg5 and vtg2. The loss of vtgencoding genes in placental mammals is consistent with the gain. Introduction vitellogenin or vtg or vgtaken from latinvitelluswhich means yolk and gener means to produce is anidentical term for the gene and the uttered protein. Vitellogenin assay by enzymelinked immunosorbant assay as. Estrogen efficiently induced vtg in the eyesac lymph of goldfish. Vitellogenesis the synthesis and storage of yolk in the developing female sexual cells of animals oocytes during oogenesis. They must also choose between using the vitellogenin gene or the protein. Our series, animals in research, profiles the top organisms used for science experimentation. Revealing genes associated with vitellogenesis in the.
In studies of sex discrimination in fish in the early 1900s, a specific antigen in the blood of gravid females was identified using immunological methods. The transcription of the femalespecific vitellogenin was significantly induced in testes. Sensitivity of the vitellogenin assay to diagnose exposure. Additionally, similar evolutionary patterns were observed in the vtg genes from other invertebrate and vertebrate organisms, suggesting that duplication, concerted evolution and purifying selection may be the major evolutionary forces driving vtg gene evolution across highly divergent taxa. Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic edcs. It was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific dna sequences on chromosomes. A zebrafish vitellogenin gene vg3 encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene author links open overlay panel hai wang tie yan jacqueline t. Revealing genes associated with vitellogenesis in the liver. Identification and expression analysis of vitellogenin. Wikigenes vtg1 vitellogenin 1 collaborative publishing. Previous studies focused on a relatively small number of hepatic expressed genes during vitellogenesis. The use of vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic endocrine disrupting. Molecular cloning of vitellogenin gene and mrna expression by 17aethinylestradiol from slender.
Differential gene expression analysis in fish exposed to endocrine disrupting compounds. Vitellogenin assay by enzymelinked immunosorbant assay as a. Characterization and expression profile of vitellogenin gene. Venema points out, is produced by a gene called vitellogenin. Proteomics analysis of early developmental stages of zebrafish. Vitellogenic definition of vitellogenic by the free. After fitclabeled vtg was prepared, it was injected into mature. Vitellogenin is frequently used as a biomarker of exposure to environmental estrogens. Vitellogenin is normally secreted into the hemolymph of reproductive females before transport into. All resources generated by the zgc are publicly accessible to the biomedical research community. The zebrafish reference genome sequence and its relationship.
Fluorescent zebra fish were specially bred to help detect environmental pollutants. Vitellogenin is the precursor of two yolk proteins. Teco multispecies vitellogenin elisa kit can be used to assay vitellogenin in the serum, whole body homogenate and mucus of numerous marine and freshwater fish species. Red zebra danio glofishglow in the dark fwf danios zebra red fish size. The value of zebrafish as an integrative model in effect. The vtg gene and protein were induced by ee2 exposure in liver, skin and eye tissues of male zebrafish in dose and timedependent patterns. Vitellogenin is the precursor of the lipoproteins and phosphoproteins that make up most of the protein content of yolk. Pdf our understanding of the functions of vitellogenin vtg in reproduction has. Here we report an alignment of the amino acid sequences of vitellogenins from frog and chicken with those from threec. Vitellogenin is an acute phase protein with bacterialbinding and. Lucey university of massachusetts amherst follow this and additional works at. A zebrafish vitellogenin gene vg3 encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene. In oviparous vertebrates, including fish, vitellogenesis consists of highly regulated pathways involving 17.
Aug 15, 2005 read hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, danio rerio, gene on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. We attempted to induce vitellogenin vtg in the eyesac lymph of bubbleeye goldfish and develop a method for visualizing vtg incorporation by zebrafish oocytes using fitclabeling. Characteristics of fish yolk proteins and a method for inducing vitellogenin sean m. May 15, 20 read characterization and expression profile of vitellogenin gene from scylla paramamosain, gene on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. A zebrafish vitellogenin gene vg3 encodes a novel vitellogenin without a phosvitin domain and may represent a primitive. Larvae are placed into tanks with little to no water exchange to start, and increasing water flow as the fish improve their ability to hunt and swim. A highquality sequence assembly of the zebrafish genome reveals the largest gene set of any vertebrate and provides information on key genomic features, and comparison to. Oecd test guideline 229 fish shortterm reproduction assay oecd test guideline 230 21day fish assay oecd test guideline 234 fish sexual development test the biosense vitellogenin elisa kits have been developed for the standard fish test species recommended by oecd, and are used in many ecotoxicology laboratories throughout the world. Distribution of vitellogenin in zebrafish danio rerio. Loss of egg yolk genes in mammals and the origin of lactation. The liver synthesizes and secretes vitellogenin, which is carried in the bloodstream to the oocytes.
Vitellogenin as a biomarker for xenobiotic estrogens in an amphibian model system. Differential immune response of vitellogenin gene to vibrio anguillarum in noble scallop chlamys nobilis and its correlation with total carotenoid content. In zebrafish, seven vtg genes were previously identified 25 but. When designing an experiment using vitellogenin, investigators must decide whether to deploy fish in the field or expose fish in the lab, how many fish to expose, and how long to expose the fish.
Tan jt, gong z 2000 a zebrafish vitellogenin gene vg3 encodes a novel vitellogenin without a phosvitin domain. Vitellogenin acts as the carrier molecule for materials for the developing egg. This study mainly shows the dna sequence and expression analysis of vitellogenin in actias selene ash vg. Meanwhile, bioassays with zebrafish, an established model organism in different research areas, are increasingly being utilized in eda. Cg31150 resembles other vg family genes in having an nterminal vitellogenin n domain, duf1943. Periodic mating keeps eggs healthy, but making all those eggs takes a lot of work, so individual females should only be mated once per week. Caenorhabditis elegans vitellogenins are encoded by a family of six genes, one of which,vit5, has been previously sequenced and shown to be surprisingly closely related to the vertebrate vitellogenin genes. Read a zebrafish vitellogenin gene vg3 encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene, gene on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The loss of vtg encoding genes in placental mammals is consistent with the gain. Apr 17, 20 a highquality sequence assembly of the zebrafish genome reveals the largest gene set of any vertebrate and provides information on key genomic features, and comparison to the human reference. They found that while monotremes still have one functional vitellogenin gene, in placental.
Vitellogenin motifs conserved in nematodes and vertebrates. Queen bees naturally vaccinate their offspring biology. Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity. Cg31150 resembles other vg family genes in having an n. Fish display relatively high variability in vitellogenin features compared with other vertebrates and even within salmonids, molecular weight may range from 342 to 600 kda mommsen and walsh 1988. May 08, 20 our series, animals in research, profiles the top organisms used for science experimentation. Vitellogenin is a protein occurring naturally in the serum of adult female nonmammalian vertebrates during egg production. Vitellogenin vtg is known to be a femalespecific protein and a. In the presence of estrogenic endocrine disruptive chemicals edcs, male fish can express the vg gene in a dose dependent manner. Suitable for use with japanese rice fish, rainbow fish and up to 90% of marine fish species including atlantic cod, common dab, european plaice, and european flounder. Gene 2000 vitellogenin expression in zebrafish danio rerio.
Follicle growthvitellogenesis, vtgs, decreased hatching rate and survival. Zebrafish undergo sexual reproduction, so breeding requires both male and female fish. Zebrafish are probably not the first creatures that come. Vitellogenin vg is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic edcs, male fish can express the vg gene but in a dose dependent manner. Read characterization and expression profile of vitellogenin gene from scylla paramamosain, gene on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. A growing concern over endocrine disruption in aquatic species has prompted the development of molecular assays to monitor environmental impacts. This study aims to identify hepatic genes involved in vitellogenesis and regulated by e2, by using zebrafish microarray gene expression profiling, and to provide information on functional distinctive genes expressed in the liver of a vitellogenic female, using zebrafish as a model fish. Duplication, concerted evolution and purifying selection. Disruption of vitellogenin gene function in adult honeybees. Hepatic and extrahepatic expression of vitellogenin genes in.
Sex differences in the zebrafish transcriptome have been studied. Zebrafish danio rerio, a small teleost fish belonging to the family cyprinidae, is widely used as a model organism in developmental biology and endocrinology. At present, this specific antigen is known as vitellogenin, the major precursor of egg yolk protein that is synthesized in the female liver and is secreted into the blood to be incorporated into the egg. After fitclabeled vtg was prepared, it was injected into mature female zebrafish. They found that while monotremes still have one functional vitellogenin gene, in. Sensitivity of the vitellogenin assay to diagnose exposure of. Males have slimmer bodies with a slightly red hue, while females have bigger, silver bellies full of eggs. Jan 20, 2003 the vitellogenin gene was chosen because its expression is unlikely to have a phenotypic effect until the adult stage in bees.
Read hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, danio rerio, gene on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Mar 19, 2008 loss of egg yolk genes in mammals and the origin of lactation and placentation date. The yolk protein precursor vitellogenin vtg has proved to be a simple and sensitive biomarker for assessing exposure of fish to edcs, especially the estrogenic compounds. Work is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals, and in the focus of this development are the fish test species. This study aims to identify hepatic genes involved in vitellogenesis and regulated by e2, by using zebrafish microarray gene expression profiling, and to. The vitellogenin gene was chosen because its expression is unlikely to have a phenotypic effect until the adult stage in bees. Vitellogenin receptor and neuropeptide receptors involved in reproduction of the red imported fire ant solenopsis invicta buren a dissertation. And recently some researchers decided to find out if humans carry the pseudogene for vitellogenin. The decrease of estradiol concentration in different fish has.
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